The Bieshikent Depression at the right bank of Amu Darya in Turkmenistan is characterized by low degree of exploration and lack of geologic knowledge. Based on core and thin section observation, combined with the analysis of physical properties and logging interpretation, the methods such as crossplot and electrofacies analysis were applied to study the lithologies, physical properties, reservoir spaces, reservoir types and main controlling factors of reservoir development of limestone reservoir of Callovian-Oxfordian. The results show that the reservoir rocks of Callovian-Oxfordian are mainly composed of micrite limestones and micrite granular limestones, and micro sparite granular limestones come second. The reservoir physical properties are poor, with low porosity and low-medium permeability. The main reservoir spaces are secondary dissolved pores, with some micro fractures and a small amount of biological cavity hole, so it mainly belongs to porous-fracture reservoir. The reservoir development is mainly controlled by sedimentary facies. The favorable reservoirs are mainly developed in microfacies such as bioclastic beach, bioclastic mound and grannular mound, with a little in lime mound. Dissolution and tectonic fracturing improve the reservoir properties. This study results can provide a reference for further exploration and development of Callovian-Oxfordian reservoir.