辽河高凝油微生物采油菌剂研究及应用评价

doi:10.3969/j.issn.1673-8926.2017.05.020

岩性油气藏 ›› 2017, Vol. 29 ›› Issue (5): 162–168.doi: 10.3969/j.issn.1673-8926.2017.05.020

• 石油工程 • 上一篇

辽河高凝油微生物采油菌剂研究及应用评价

王小通¹, 向龙斌², 张艺馨³

1. 中国石油勘探开发研究院, 北京 100083;
2. 中国地质大学(武汉)工程学院, 武汉 430074;
3. 密苏里科技大学罗拉分校, 密苏里州罗拉 65401

收稿日期:2016-12-21 修回日期:2017-02-15 出版日期:2017-09-21 发布日期:2017-09-21
第一作者:王小通(1987-),男,中国石油勘探开发研究院在读博士研究生,研究方向为微生物提高原油采收率及微生物技术应用。地址:(100083)北京市海淀区学院路20号中国石油勘探开发研究院。Email:jacker_orange@126.com。
通信作者: 向龙斌(1964-),男,博士,教授,主要从事石油勘探开发与资源型企业管理方面的教学与研究工作。Email:xianglb@cug.edu.cn。
基金资助:
国家高技术研究发展计划（863计划）项目“中低温油藏微生物定向激活与模拟技术研究”（编号：2013AA064402）资助

Microorganism preparation and application evaluation on microbial enhanced high-pour point oil recovery in Liaohe Oilfield

WANG Xiaotong¹, XIANG Longbin², ZHANG Yixin³

1. PetroChina Research Institute of Petroleum Exploration and Development, Beijing 100083, China;
2. Faculty of Engineering, China University of Geosciences, Wuhan 430074, China;
3. Missouri University of Science and Technology at Rolla, Rolla 65401, Missouri, U.S.A

Received:2016-12-21 Revised:2017-02-15 Online:2017-09-21 Published:2017-09-21

摘要/Abstract

摘要： 针对高凝油含蜡高、凝固点高、流动性差及开采难度大的问题，选用铜绿假单胞菌配合嗜热脂肪地芽孢杆菌和嗜热脱氮地芽孢杆菌，采用四组分分析法和饱和烃气相色谱法等方法开展了微生物提高高凝油采收率菌剂研究和应用评价。结果表明：菌种对原油四组分存在选择性降解，降解率为23.0%～42.3%，同时菌种可以将高凝油中长碳链饱和烃降解为短碳链烃类，w（nC_21^-）/w（nC_22⁺）值和w（nC₂₁＋nC₂₂）/w（nC₂₈+nC₂₉）值增大0.33～0.57；铜绿假单胞菌发酵液表面张力从72.21mN/m降低至26.81mN/m；嗜热脂肪地芽孢杆菌与嗜热脱氮地芽孢杆菌2种芽孢杆菌乳化高凝油的E24值分别为70.6%和82.3%；基于嗜热脂肪地芽孢杆菌、嗜热脱氮地芽孢杆菌和铜绿假单胞菌3种细菌性能设计的兼容本源微生物的复合微生物采油菌剂可使高凝油黏度降低63.86%，凝固点降低6℃。物理模拟驱油实验表明：该微生物复合菌剂可在中渗（200mD）及低渗（50mD）条件下使高凝油采收率提高6.46%～8.48%。6口油井的微生物吞吐采油试验证明该微生物复合菌剂性能稳定，可使高凝油采收率大幅提高，具有良好的工业应用前景。

关键词: 高分辨率层序地层学, 基准面, 冲积扇, 馆陶组, 辽河坳陷

Abstract: The high pour-point oil is characterized by high proportion of wax,high freezing point and low fluidity, which make it difficult for development. To solve this problem,one strain of P.aeruginosa and two strains of Geobacillus were selected for microbial enhance high-pour point oil recovery research and characteristics assessment through four components analysis and the saturated hydrocarbon GC spectrum analysis. The results show that four components of oil selectively degrade by the strains:degradation rate is 23.0%-42.3%,meaning while the long carbon chain in the oil is shortened,the value of w(nC_21^-)/w(nC_22⁺) and w(nC₂₁ + nC₂₂)/w(nC₂₈ + nC₂₉) increased by 0.33-0.57. The strains'capability of producing bio-surfactant was evaluated:the surface tension of P.aeruginosa fermentation broth decreases from 72.21 mN/m to 26.81 mN/m. The two strains of Geobacillus emulsify high-pour point oil with an emulsification index of 70.6% and 82.3% respectively. Based on characteristics of these three microorganisms,a novel compound microorganism preparation which is compatible with indigenous microorganisms was successfully designed. The oil viscosity decreases by 63.86% and the oil freezing point is lowered by 6℃ by this microbial preparation. Through water flooding simulation test,the preparation enhances high-pour point oil recovery by 6.46%-8.48% in the middle permeability(200 mD) and low permeability(50 mD). Afield test involved 6 wells was conducted in Liaohe Oilfield,which demonstrates the stability and high efficiency of this novel compound microorganism preparation in enhancing high freezing point oil recovery.

Key words: high-resolution sequence stratigraphy, base level, alluvial fan, Guantao Formation, Liaohe Depression

中图分类号:

TE357.9

王小通, 向龙斌, 张艺馨. 辽河高凝油微生物采油菌剂研究及应用评价[J]. 岩性油气藏, 2017, 29(5): 162–168.

WANG Xiaotong, XIANG Longbin, ZHANG Yixin. Microorganism preparation and application evaluation on microbial enhanced high-pour point oil recovery in Liaohe Oilfield[J]. Lithologic Reservoirs, 2017, 29(5): 162–168.

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辽河高凝油微生物采油菌剂研究及应用评价

Microorganism preparation and application evaluation on microbial enhanced high-pour point oil recovery in Liaohe Oilfield

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